PTH- amino acid

conditions;

column: Develosil XG-C18LC(5um) 4.6x150mm
Mobile phase :A) 0.2% Phosphoric acid - Water
                    B) 0.2% Phosphoric acid - Acetonitrile
Flow rate : 1.0ml/min
Temperature : 40℃
Detection : UV254nm
Sample : 1: PTH-Histidine
             2: PTH-Arginine
             3: PTH-Aspartic acid
             4: PTH-Glutamic acid
             5: PTH-Methionine
             6: PTH-Tryptophan
             7: PTH-Phenylalanine
             8: PTH-Leucine
System : Thermo Scientific ultimate 3000 series